Multi-Scale Biosurfactant Production by Bacillus subtilis Using Tuna Fish Waste as Substrate

As one of the most effective biosurfactants reported to date, lipopeptides exhibit attractive surface and biological activities have great potential serve as biocatalysts. Low yield, high cost production, purification hinder large-scale applications lipopeptides. Utilization waste materials low-cost substrates for growth biosurfactant producers has emerged a feasible solution economical production. In this study, fish peptone was generated through enzyme hydrolyzation smashed tuna (Katsuwonus pelamis). Biosurfactant (mainly surfactin) production by Bacillus subtilis ATCC 21332 further evaluated optimized using comprehensive substrate. The conduction continuously assessed in 7 L batch-scale 100 pilot-scale fermenter, exploring possibility surfactin results showed that could effectively use peptones highest productivity achieved experiments 274 mg/L. experimental shed light on surfactins at scales wastes an